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Six-week-old female BALB/c nude mice were purchased from Shanghai Laboratory Animal Center, Chinese Academy of Sciences and Technology (Shanghai, China). All animals were housed and maintained in specific pathogen-free conditions according to the recommendation of Guide for the Care and Use of Laboratory Animals of the National Institutes of Health with strict accordance with protocols approved by the Institutional Animal Care and Use Committee of Guizhou Medical University.
All the human colorectal cancer specimens were collected in Affiliated Hospital of Guizhou Medical University. The use of patient samples was approved by the Ethics Committee of Guizhou Medical University (Approval no. 2018-123-01) and informed consent was obtained from the patients. All animals were housed and maintained in specific pathogen-free conditions according to the recommendation of Guide for the Care and Use of Laboratory Animals of the National Institutes of Health with strict accordance with protocols approved by the Ethics Committee of Guizhou Medical University (Approval no.1900628)
Transfected BCa cells were seeded in 96-well chamber (CLS3374; Corning, Cambridge, MA, USA). The upper chambers were supplemented with serum-free medium and the lower ones were with complete medium. For invasion assays, the upper chambers were precoated with Matrigel (#356234; BD Biosciences, San Jose, CA, USA) particularly. After 24 h of incubation, the chambers were fixed in methanol and then stained by crystal violet. The migrated or invaded cells were photographed under a light microscope. Each experiment was repeated three times and three replicates for each bio-repeat. 153554b96e
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